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Guanfeng Lin Tiancai Liu Jingyuan Hou Zhiqi Ren Jianwei Zhou Qianni Liang Zhenhua Chen Wenqi Dong Yingsong Wu 《Journal of fluorescence》2015,25(2):361-367
A sensitive, rapid and novel measurement method for cytokeratin 19 fragment (CYFRA 21–1) in human serum by magnetic particle-based time-resolved fluoroimmunoassay (TRFIA) is described. Built on a sandwich-type immunoassay format, analytes in samples were captured by one monoclonal antibody coating onto the surface of magnetic beads and “sandwiched” by another monoclonal antibody labeled with europium chelates. The coefficient variations of the method were lower than 7 %, and the recoveries were in the range of 90–110 % for serum samples. The lower limit of quantitation of the present method for CYFRA 21–1 was 0.78 ng/ml. The correlation coefficient of CYFRA 21–1 values obtained by our novel TRFIA and CLIA was 0.980. The present novel TRFIA demonstrated high sensitivity, wider effective detection range and excellent reproducibility for determination of CYFRA 21–1 can be useful for early screening and prognosis evaluation of patients with non-small cell lung cancer. 相似文献
94.
M. Vogel G. Birkl M. S. Ebrahimi D. von Lindenfels A. Martin G. G. Paulus W. Quint S. Ringleb Th. Stöhlker M. Wiesel 《Hyperfine Interactions》2015,230(1-3):65-71
We present two Penning trap experiments concerned with different aspects of the physics of extreme electromagnetic fields, the ARTEMIS experiment designed for bound-electron magnetic moment measurements in the presence of the extremely strong fields close to the nucleus of highly charged ions, and the HILITE experiment, in which well-defined ion targets are to be subjected to high-intensity laser fields. 相似文献
95.
96.
Mark Weinstein 《Foundations of Chemistry》2016,18(3):195-212
The periodic table may be seen as the most successful example of inquiry in the history of science, both in terms of practical application and theoretic understanding. As such, it serves as a model for truth as it emerges from inquiry. This paper offers a sketch of a central moment in the history of chemistry that illustrates an intuitive metamathematical construction, a model of emerging truth (MET). The MET, reflecting the structure the surrounds the periodic table, attempts to capture the salient epistemological elements that warrant truth claims based on sets of models that are progressive in light of both empirical and theoretical advance seen over time. 相似文献
97.
Nadia Lahrichi Teodor Gabriel Crainic Michel Gendreau Walter Rei Louis-Martin Rousseau 《The Journal of the Operational Research Society》2015,66(1):44-56
The dairy transportation problem (DTP) consists of determining the best routes to be performed for collecting milk from farms and delivering it to processing plants. We study the particular case of the province of Quebec, where the Fédération des producteurs de lait du Québec is responsible for negotiating the transportation costs on behalf of producers. Several issues are highlighted in the actual process of designing contracts such as using historical data. We propose an approach based on scenario analysis that consists of revising both the steps and the information used to construct the routes. We develop a generalized tabu search algorithm that integrates the different characteristics of the DTP. 相似文献
98.
Yuan Hu Brad Sherborne Tai-Sung Lee David A. Case Darrin M. York Zhuyan Guo 《Journal of computer-aided molecular design》2016,30(7):533-539
In drug discovery, protonation states and tautomerization are easily overlooked. Through a Merck–Rutgers collaboration, this paper re-examined the initial settings and preparations for the Thermodynamic Integration (TI) calculation in AMBER Free-Energy Workflows, demonstrating the value of careful consideration of ligand protonation and tautomer state. Finally, promising results comparing AMBER TI and Schrödinger FEP+ are shown that should encourage others to explore the value of TI in routine Structure-based Drug Design. 相似文献
99.
Rob G. Visser 《Accreditation and quality assurance》2016,21(3):237-238
The tentative arguments mentioned by Paul De Bievre are discussed and alternatives are given that may lead to different insights. 相似文献